Carboxyfluorescein Diacetate Succinimidyl Ester (CFDA SE) is a colorless, non-fluorescent, stable cell-permeable dye. Once it enters into the cells, the intracellular esterases remove the acetate groups to generate Carboxyfluorescein Succinimidyl Ester, which then covalently binds to lysine residues or other amines and produces a bright fluorescent conjugate. The fluorescent conjugate is stable and is retained inside the cells during cell division and hence it is an ideal tool for cell tracing studies, both in vitro and in vivo.
The EZCell™ CFDA SE Cell Tracer Kit provides an easy, rapid and sensitive way to determine cell proliferation using CFDA SE dye. The dye can be inherited by the daughter cells for up to 8 generations. Following each cell division, the fluorescence intensity is halved, which can be quantified by measuring the fluorescence intensity at Ex/Em = 492/517 nm. The assay is non-radioactive, accurate and can be used for cell tracing studies.
Supplier: BioVision
Catalogue number: K930-100
Size: 100 µl
The Cell Stimulation Cocktail (500X) is a ready-to-use cocktail useful to induce activation of many cell types to produce cytokines. This cocktail can be used to induce cytokine production in in vitro cell culture for subsequent detection in immunoassays. Each vial contains Protein Kinase C activator Phorbol 12-Myristate 13-Acetate (PMA) (40.5 µM) and the calcium ionophore, Ionomycin (670 µM) formulated in DMSO (500X).
Product Details
Appearance
Liquid
Formulation
In DMSO
CAS #
See under individual products
Molecular Formula
See under individual products
Molecular Weight
See under individual products
Handling
Protect from air and light
Storage Conditions
-20°C
Shipping Conditions
Gel Pack
USAGE
For Research Use Only! Not For Use in Humans.
biovision ezcell cocktail
Description
The Cell Stimulation and Protein Transport Inhibitor Cocktail is a ready-to-use cocktail consisting of an optimized mixture of Phorbol 12-Myristate 13-Acetate (PMA), Ionomycin, Brefeldin A, and monesin. Formulated in DMSO (500X). PMA and Ionomycin in duce cytokine production in many cell types and Brefeldin A and Monensin act as protein transport inhibitors. This cocktail is used to induce cytokine production and inhibit their intracellular transport for flow cytometry detection.
The EZCell Protein Transport Inhibitor Cocktail (500X) is a cocktail composed of of Brefeldin A and Monensin, formulated in DMSO. Useful in assays that require prevention of protein transport to the extracellular space is required. Addition of this cocktail to cells in culture inhibits the intracellular protein secretory/transport pathway resulting in the accumulation of secreted proteins in the lumen of the endoplasmic reticulum and in the Golgi apparatus. This accumulation can then be detected by intracellular staining and flow cytometric analysis or Western blotting.
More Information
SKU
K932-500
Size
500 ul
Application
A Protein Transport Inhibitor cocktail
Cas no
See under individual products
Storage
-20C
Shipping Temp
Gel Pack
Alternate Name : EZCell™ Cell Tracer Kit,CFDA Cell Tracer Kit,CFDA SE Cell Tracer Kit,EZCell™ CFDA SE Cell Tracer Kit,EZCell™ CFDA SE Cell Tracer Kit,EZCell™ CFDA SE Cell Kit,Cell Tracer Kit
Tag Line : Flow cytometry based assay to determine cell proliferation using CFDA SE dye
Summary : BioVisions EZCell™ CFDA SE Cell Tracer Kit provides an easy, rapid and sensitive way to determine cell proliferation using CFDA SE dye. The dye can be inherited by the daughter cells for up to 8 generations. Following each cell division, the fluorescence intensity is halved, which can be quantified by measuring the fluorescence intensity at Ex/Em = 492/517 nm. The assay is non-radioactive, accurate and can be used for cell tracing studies.
