Product name: Alpha-Ketoglutarate (Alpha KG) Assay Kit
Detection method: Colorimetric / Fluorometric
Example type: Tissue, adherent cells, cells in suspension
Test type: Enzyme activity (quantitative)
Sensitivity: = 0.01 nmol / well
Distance: 0.01 nmol / well – 10 nmol / well
Test time: 0 h 30 min
The Alpha-Ketoglutarate (Alpha KG) Assay Kit provides a simple, sensitive, and rapid means of quantifying alpha KG. In the alpha KG assay protocol, alpha-ketoglutarate is transaminated with the generation of pyruvate, which is used to convert a nearly colorless probe into a colored (λmax = 570 nm) and fluorescent (Ex / Em = 535/587) product. nm).
Summary of Alpha-Ketoglutarate Assay Protocol:
– add the reaction mix to the sample and standard wells
– incubate for 30 min
– analyze with a microplate reader
Abcam has not applied for and does not intend to apply for REACH Authorization for the use of products by customers containing substances on the European Authorization List (Annex XIV). It is the responsibility of our clients to verify the need to apply the REACH Authorization, and any other relevant authorization, for the intended uses.
Alpha-ketoglutarate (Alpha-KG) is a key intermediate in the Krebs cycle, which comes after isocitrate and before succinyl CoA. Anaplerotic reactions replenish the cycle by synthesizing Alpha-KG from glutamate transamination or through the action of glutamate dehydrogenase. Alpha-KG is an important nitrogen transporter. Being a key intermediate, it is one of the organic acids measured in newborns as an indicator of innate errors of metabolism.