Alpha-Ketoglutarate Assay Kit 100 assays

Alpha-Ketoglutarate Assay Kit 100 assays

Product name: Alpha-Ketoglutarate (Alpha KG) Assay Kit

Detection method: Colorimetric / Fluorometric

Example type: Tissue, adherent cells, cells in suspension

Test type: Enzyme activity (quantitative)

Sensitivity: = 0.01 nmol / well

Distance: 0.01 nmol / well – 10 nmol / well

Test time: 0 h 30 min

Product description

The Alpha-Ketoglutarate (Alpha KG) Assay Kit provides a simple, sensitive, and rapid means of quantifying alpha KG. In the alpha KG assay protocol, alpha-ketoglutarate is transaminated with the generation of pyruvate, which is used to convert a nearly colorless probe into a colored (λmax = 570 nm) and fluorescent (Ex / Em = 535/587) product. nm).

Summary of Alpha-Ketoglutarate Assay Protocol:
– add the reaction mix to the sample and standard wells
– incubate for 30 min
– analyze with a microplate reader

Notes

Abcam has not applied for and does not intend to apply for REACH Authorization for the use of products by customers containing substances on the European Authorization List (Annex XIV). It is the responsibility of our clients to verify the need to apply the REACH Authorization, and any other relevant authorization, for the intended uses.

Relevance

Alpha-ketoglutarate (Alpha-KG) is a key intermediate in the Krebs cycle, which comes after isocitrate and before succinyl CoA. Anaplerotic reactions replenish the cycle by synthesizing Alpha-KG from glutamate transamination or through the action of glutamate dehydrogenase. Alpha-KG is an important nitrogen transporter. Being a key intermediate, it is one of the organic acids measured in newborns as an indicator of innate errors of metabolism.

Alternative names

Alpha KG

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